Several chemokines express in spinal cord after the injury. They increase leukocyte infiltration and are implicated in the inflammatory responses. Although inflammatory control is very important for prevention and resolution after the spinal cord injury (SCI), attitude of the chemokines and their receptors have not been understood in detail. Male C57/BL6 mice were subjected to spinal cord transection by razor at level between T9 and T10 intervertebral spinal cord. The spinal cord from T8 to T11 was then collected on sham-operated control mice (day 0), and on post-operative day 1, 3, 7 and 14. The tissues were quantified gene expressions of monocyte/macrophage-related CC- and CXC-chemokines, and the receptors by SYBR-Green based qPCR. Moreover, the frozen sagittal sections of spinal cord were immunostained against CCL2, CCL5 and the receptor antibodies on day 0, 1 and 14. The gene expression of the chemokines was divided into two groups: increase transiently within 3 days after SCI such as CCL2 and CXCL1, and increase progressively for 14 days such as CCL5. Immunoreactivities of CCL2, and CCL5 were observed in the spinal cord at day 1 and were merged with a neuronal marker, NeuN. Although the CCL2 immunoreactivity was diminished at day 14, the CCL5 immunoreactivity was merged with microglial/macrophage and astroglial markers, Iba1 and GFAP-positive reactions in the peri-injury site as well. At day1, CCR2 and CCR5 immunoreactions were merged mainly with a neutrophil marker, Ly-6G positive cells and partially with Iba1 positive cells. CCR2 immunoreactivity was decreased at day 14. CCR5 immunoreactivity was recognized in Iba1 and GFAP-positive cells in the peri-injury site. In present study, we determined acute and chronic expressed monocyte/macrophage-related chemokines after the SCI. Moreover, we determined localization and type of cells expressing CCL2, CCL5 and the receptors. We have been examining the function of cells expressing the receptors with immunohistochemistry (COI: No).