17:00 - 19:00
Room: ANA Crowne Plaza “Ohtori” Room A
Evening Symposium
Chair/s:
Naofumi Mukaida, Dhan V. Kalvakolanu
Chemokine-dependent and -independent mechanisms of T cell immune surveillance
Jens V. Stein
Theodor Kocher Institute, University of Bern, Bern, Switzerland

Rapid migration of naïve T cells through lymph node (LN) parenchyma and non-lymphoid tissue (NLT) is critical for detection of antigens and effective immune responses. Here we use in vitro and in vivo imaging to dissect the mechanics of naïve T cell migration in LN and determine how chemokine receptor CCR7, integrin LFA-1 and cortical actin contribute to intranodal locomotion. We find that T cells undergo periodic shape oscillations, where elongated shapes coincide with forward translocation and increased actin flow, which is quantitatively tuned by the strength of chemokine signaling. LFA-1 does not control cytoskeletal dynamics but creates tangential friction between actin cortex and environment, without adhesively confining the cells to lymph node stroma. In contrast to naïve T cells, we found that memory T cells migrating through NLT, such as salivary glands, do not require chemokines for efficient F-actin treadmilling or integrin coupling. Rather, integrin-independent intrinsic cell shape changes are sufficient and necessary for T cell surveillance of epithelial and non-epithelial compartments. The unexpectedly distinct dependencies of naïve and memory T cells on chemokines and integrins for efficient immune surveillance probably reflect the homogeneous structure of lymphoid organs versus the highly divergent NLT structure in terms of physical and biochemical properties.


Reference:
Tu-ES4-4
Session:
Evening Symposium “Chemokines ---- Cell trafficking and beyond”
Presenter/s:
Jens V. Stein
Presentation type:
Invited Speaker
Room:
ANA Crowne Plaza “Ohtori” Room A
Chair/s:
Naofumi Mukaida, Dhan V. Kalvakolanu
Date:
Tuesday, 31 October 2017
Time:
18:12 - 18:36
Session times:
17:00 - 19:00