Immunological memory is important for vaccination and suppression of neoplasia, however, it is still unclear how memory function is maintained for a long period. We have established an in vitro reprogaming method that induces memory T cells with naive-like phenotypes (iTscm cells) from activated T cells. We found that both CD4+ and CD8+ activated effector T cells, both from mouse and human, could become iTscm cells by co-culturing them with Notch-ligand expressing stroma cells. iTscm cells not only supplied classical central and effector memory T cell populations (Tcm and Tem) by antigen re-stimulation, but also possessed long-lived and self-renewing capacities. iTSCM suppressed tumor growth much more efficiently than Tcm or Tem cells. Our data suggest that Notch signaling confers “stemness” on effector T cells, and converts them into potent anti-tumorigenic T cells. We have investing the mechanism of iTscm induction by the Notch signaling, and found that metabolic reprograming is important fro the generation of iTscm cells.