19:10 - 21:00
Room: Ishikawa Ongakudō Interchange Hall
Poster Session
Microbially cleaved immunoglobulins are sensed by the innate immune receptor LILRA2
Kouyuki Hirayasu1, Fumiji Saito2, Tadahiro Suenaga1, 2, Kyoko Shida1, Noriko Arase2, 3, Keita Oikawa4, Toshifumi Yamaoka3, Hiroyuki Murota3, Hiroji Chibana5, Ichiro Nakagawa6, Tomoko Kubori7, Hiroki Nagai7, Yuji Nakamaru8, Ichiro Katayama3, Marco Colonna9, Hisashi Arase1, 2
1Laboratory of Immunochemistry, WPI Immunology Frontier Research Center, Osaka University, Suita, Japan, 2Department of Immunochemistry, Research Institute for Microbial Diseases, Osaka University, Suita, Japan, 3Department of Dermatology, Graduate School of Medicine, Osaka University, Suita, Japan, 4Department of Otolaryngology, Tenshi Hospital, Sapporo, Japan, 5Medical Mycology Research Center, Chiba University, Chuo-ku, Japan, 6Department of Microbiology, Kyoto University Graduate School of Medicine, Kyoto, Japan, 7Laboratory of Combined Research on Microbiology and Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Japan, 8Department of Otolaryngology-Head and Neck Surgery, Hokkaido University Graduate School of Medicine, Sapporo, Japan, 9Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, United States

Microbial proteases degrade a variety of host proteins. However, it has remained largely unknown why microbes have evolved to acquire such proteases and how the host responds to microbially degraded products. Here, we found that immunoglobulins disrupted by microbial pathogens are specifically detected by leukocyte immunoglobulin-like receptor A2 (LILRA2), an orphan activating receptor expressed on human myeloid cells. Proteases from M. hyorhinis, L. pneumophila, and C. albicans cleaved N-terminus of immunoglobulins. Identification of the immunoglobulin-cleaving protease from L. pneumophila revealed that the protease is conserved across some bacteria. These microbially cleaved immunoglobulins but not normal immunoglobulins stimulated human neutrophils and induced IL-8 via LILRA2. In addition, stimulation of primary monocytes via LILRA2 inhibited the growth of L. pneumophila. When mice were infected with L. pneumophila, immunoglobulins were cleaved and recognized by LILRA2. More importantly, cleaved immunoglobulins were detected in the patients with bacterial infections and stimulated LILRA2-expressing cells. Our findings demonstrate that LILRA2 is a type of innate immune receptors in the host immune system to detect immunoglobulin abnormalities caused by microbial pathogens.


Reference:
Mo-P1-38
Session:
Poster Session 1 ‟Innate immunity and infection”
Presenter/s:
Kouyuki Hirayasu
Presentation type:
Poster Presentation
Room:
Ishikawa Ongakudō Interchange Hall
Date:
Monday, 30 October 2017
Time:
19:10 - 21:00
Session times:
19:10 - 21:00