19:10 - 21:00
Poster Session 1 ‟Innate immunity and infection”
Room: Ishikawa Ongakudō Interchange Hall
Poster Session
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Mycobacterium tuberculosis Rv0351 exhibits vaccine potential against the highly-virulent Beijing K strain: Interaction with dendritic cells, Th1 immunity generation, immune sensing by T cells, and maintenance of multifunctional T cells
Woo Sik Kim, Jong-Seok Kim, Kee Woong Kwon, Hongmin Kim, Sung Jae Shin
Department of Microbiology, Institute for Immunology and Immunological Diseases, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, South Korea, Seoul, Korea, Republic of (South)

Effective vaccination is the only cost-effective measure to achieve long-term control of tuberculosis (TB) prior to drug treatment and diagnostic test. Obviously, the first step in TB vaccine development involves vaccine target antigens assuring immunogenicity and efficacy in preclinical animal testing. In this study, Rv0351, a cofactor of heat-shock protein 70 (Hsp70), was characterized through the interplay with dendritic cells (DCs) at molecular level and evaluated as a subunit vaccine by comparing with Hsp70 against the hyper-virulent Mycobacterium tuberculosis (Mtb) Beijing K-strain. Rv0351-immunization significantly induced multifunctional CD4+ T cells co-expressing IFN-γ, TNF-α, and IL-2 in lungs from Mtb K-infected mice, whereas Rv0350-immunization did mixed Th1/Th2 immune responses. Rv0351-immunization conferred a significant protection while a negligible protective efficacy was observed with Rv0350-immunized mice. To address how Rv0351 generated the protective immunity, its molecular characteristics was determined by the interaction with DCs. Rv0351 showed an excellent capacity to activate DCs along with effective polarization and differentiation of naïve CD4+ and CD8+ T cells to secrete IFN-γ and IL-2. Moreover, Rv0351-stimulated DCs induced the proliferation of Rv0351-specific Th1-type effector/memory CD4+/CD8+-T cells in the spleen of Mtb-infected mice in a TLR4-dependent manner. These results suggest that Rv0351 is an immune activator for DCs, enables to generate Th1-biased memory T cells with multifunctional capacity, and confers protection against the highly virulent Mtb K challenge.

Reference:
Mo-P1-46
Session:
Poster Session 1 ‟Innate immunity and infection”
Presenter/s:
Sung Jae Shin
Presentation type:
Poster Presentation
Room:
Ishikawa Ongakudō Interchange Hall
Date:
Monday, 30 October 2017
Time:
19:10 - 21:00
Session times:
19:10 - 21:00
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