The Chinese formulation Fang Feng Tong Sheng San (FFTSS) is a mixture consisting of 17 different components [1]. Previous investigations proved the activities of the formulation and of preparations [2].
12 main compounds of FFTSS’s component herbs have been tested by in vitro bioassays for different activities.
Oxidative stress plays a significant role in aging processes and in the formation of cancer. The most potent antioxidants were gallic acid (IC50 4.50±0.05 μ g/ml), luteolin (IC50 4.67±0.50 μ g/ml) and baicalein (IC50 10.02±0.25 μ g/ml) compared to the reference rutin (IC50 12.66±0.84 μ g/ml). The most potent inhibitors of the lipidperoxidase were baicalein (IC50 0.37±0.03 μ g/ml), baicalin (IC50 2.50±0.05 μ g/ml) and luteolin (IC50 4.07±0.79 μ g/ml) (reference quercetin IC50 1.17±0.05 μ g/ml). Acetylcholinesterase and butyrylcholinesterase are important in the treatment of the Alzheimer’s disease. The most effective substances for inhibiting acetylcholinesterase were gallic acid (IC50 2.46±4.50 μ g/ml) and wogonin (IC50 15.32±3.10 μ g/ml) (reference eserin (IC50 0.35±0.06 μ g/ml). The most potent inhibitor for butyrylcholinesterase was luteolin (IC50 24.82±0.96 μ g/ml). Baicalin showed a similar inhibitory activity (IC50 288.38±18.46 μ g/ml) as the α -glucosidase inhibitor acarbose (IC50 346.68±4.36 μ g/ml) which is used for the treatment of diabetes mellitus type II. Baicalein (IC50 44.27±2.31 μ g/ml) und baicalin (IC50 54.90±1.46 μ g/ml) turned out to be also potent inhibitors of tyrosinase (reference ascorbic acid IC50 36.73±0.67 μ g/ml) which may play a role in the pathogenesis of the Parkinsons’s disease.
Acknowledgements: Financial support from the BMWF (GZ 402.000/00013-WFN/6/2016) and the CACMS is gratefully acknowledged.
References
[1 ] Pharmacopoeia of the People's Republic of China, Vol. 1, Beijing: Chemical Industry Press; 2015 : 409.
[2] Brantner A, Al-Ajlani M, Zhou Y, Zhao H, Bian B. IJPSR 2017; 8 (8): 3278-3286.