16:00 - 18:00
Room: Poster Area - Poster Shed
Poster Presentation
Assignment of Immunomodulatory Compounds in Huangqi Jianzhong Decoction by Combining LC-MS Metabolomics and in-vitro Cytokine Production Assays
Xuehong Gao 1, Stafanie Nikles 1, Eva-Maria Pferschy-Wenzig 1, Marlene Monschein 1, Huiqin Zou 2, Yong Liu 2, Xiaojuan He 3, Danping Fan 4, Aiping Lu 3, 4, Kate Yu 5, Jimmy Yuk 5, Giorgis Issak 5, Rudolf Bauer 1
1 Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Universitaetsplatz 4, 8010 Graz, Austria, Graz
2 School of Pharmacy, Beijing University of Chinese Medicine, Beijing, China, Beijing
3 School of Chinese Medicine, Hong Kong Baptist University, Kowloon, Hong Kong, China, Hong Kong
4 China Academy of Chinese Medical Sciences, Institute of Basic Research in Clinical Medicine, Dongzhimennei Nanxiaojie 16, 100700 Beijing, China, Beijing
5 Waters Corporation, 5 Technology Drive, 01757 Milford, USA, Milford

Within non-communicable diseases (NCD), chronic inflammatory conditions represent one of the biggest challenges for modern medicine [1]. Traditional Chinese medicine (TCM) has been practiced over centuries to treat such diseases and has gained tremendous empirical knowledge. Huangqi Jianzhong Tang (HQJZT) is a famous TCM formula and decoction composed of Radix Astragali, Ramulus Cinnamomi, Radix et Rhizoma Gylycyrrhizae Praeparata cum Melle, Radix Paeoniae alba, Rhizoma Zingiberis recens, Fructus Jujubae and Saccharum granorum, which has been used for the treatment of various chronic inflammatory gastrointestinal diseases [2,3]. However, there is insufficient knowledge about the active compounds and mechanisms responsible for its beneficial effects.

For the analysis of the constituents of HQJZT, UHPLC- Xevo G2-XS QTOF MS based metabolomics analyses were carried out. Data processing and compound identification was performed using UNIFI software and Traditional Medicine Library. The inhibitory effects of HQJZT on TNF-α, IL-1β and IFN-γ production were studied in U937 cells. The data were correlated by multivariate data analysis (PCA, OPLS-DA) in order to identify constituents in HQJZT which may be relevant for activity.

The investigations resulted in the identification of 16 main compounds in HQJZT which are most likely contributing to its immunomodulatory and anti-inflammatory effects. Six of the compounds, namely calycosin, formononetin, astragaloside I, liquiritigenin, paeoniflorin and albiflorin were verified by LC-MS comparison with reference substances. Literature data confirmed that these compounds have anti-inflammatory effects.

References

[1] S. Prasad, B.B. Aggarwal J Clin Cell Immunol 2014, 05:238 .

[2] Y. Wei, L.-X. Ma, S.-J. Yin, J. An, Q. Wei, J.-X. Yang, Evidence-Based Complementary and Alternative Medicine 2015, 2015: 878164.

[3] Z. Zhang, X. Luo, Synopsis of prescriptions of the golden chamber with 300 cases: A classic of traditional Chinese medicine with ancient and contemporary case studies, New World Press, Beijing 1995.


Reference:
Poster Session-PO-55:
Session:
Poster Presentation-1
Presenter/s:
Xuehong Gao
Presentation type:
Poster presentation
Room:
Poster Area - Poster Shed
Date:
Monday, 27th August, 2018
Time:
16:00 - 18:00
Session times:
16:00 - 18:00