16:00 - 18:00
Room: Poster Area - Poster Shed
Poster Presentation
Modulation of iNOS Expression in LPS-Stimulated BV-2 Microglia by Prenylated Chalcones from Cullen corylifolium (L.) through Inhibition of I-κBα Degradation
Yeong Eun Han 1, Hua Li 1, Do Hee Kim 1, Ji Hye Jeong 1, Hwa Jin Lee 2, Jae-Ha Ryu 1
1 College of Pharmacy and Research Center for Cell Fate Control, Sookmyung Women’s University, Seoul
2 School of Industrial Bio-Pharmaceutical Science, Semyung University, Chungbuk

The overproduction of nitric oxide (NO) and prostaglandin E2 (PGE2) by microglia may cause neurodegenerative diseases, such as Alzheimer’s disease and Parkinson’s disease. From the activity-guided purification of Cullen corylifolium (L.) Medik. (syn. Psoralea corylifolia L.), three prenylated chalcones were identified: isobavachalcone (1), bavachromene (2), and kanzonol B (3). These prenylated chalcones showed concentration-dependent inhibitory effects on NO and PGE2 production in lipopolysaccharide (LPS)-activated microglia. Western blotting and RT-PCR analysis demonstrated that these prenylchalcones reduced the expression of protein and mRNA of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-activated microglia. Furthermore, three prenylated chalcones blocked the inhibitory-κBα (I-κBα) degradation and down-regulated nuclear factor κB (NF-κB) level of nucleus in LPS-stimulated BV-2 microglia. Therefore, these prenylated chalcones from Psoralea corylifolia may be beneficial for the treatment of neuro-inflammatory diseases by modulating iNOS and COX-2 expressions in activated microglial cells.


Reference:
Poster Session-PO-73:
Session:
Poster Presentation-1
Presenter/s:
Yeong Eun Han
Presentation type:
Poster presentation
Room:
Poster Area - Poster Shed
Date:
Monday, 27th August, 2018
Time:
16:00 - 18:00
Session times:
16:00 - 18:00