16:00 - 18:00
Room: Poster Area - Poster Shed
Poster Presentation
Improved TCM fingerprinting by benefiting from high robustness and matrix tolerance of monolithic HPLC columns
Petra Lewits 1, Dean Duan 2
1 Merck KGaA, Darmstadt
2 Merck Chemicals (Shanghai) Co., Ltd., Shanghai

When developing a new method for fingerprinting, people traditionally use one long column to obtain high peak capacity. however, long column with small particle often bring high backpressure, constitute a practical problem and limit the use of more viscous solvents in mobile phase. Monolithic column with its unique pore structure, comprising macro- and meso- pores provide lower column backpressure compared to particle packed column, even at elevated flow rate and even with more viscous mobile phase solvents, offer high peak capacity and shorter analysis running time. Besides this, robustness and resistance toward sample matrix make monolithic column to be very suitable for TCM fingerprinting. Under

Certain situation, coupling two, three and up to four Chromolith® columns in series for fingerprint analysis of herbal material has been used and published by Vander Heyden et al. in two papers.


Reference:
Poster Session-PO-143:
Session:
Poster Presentation-1
Presenter/s:
Petra Lewits
Presentation type:
Poster presentation
Room:
Poster Area - Poster Shed
Date:
Monday, 27th August, 2018
Time:
16:00 - 18:00
Session times:
16:00 - 18:00