Shiraia bambusicola is a pathogenic fungus on bamboo, whose fruiting body has been used in TCM for the treatment of vitiligo, stomachache and rheumatic in China. Hypocrellin A (HA), natural red perylenequinone pigment isolated from the fruiting body, has attracted intense interest as clinically useful photosensitizers in photodynamic therapy (PDT) for various cancers. We first analyzed the bacterial community in the fruiting body of S. bambusicola. Bacillus and Pseudomonas were the predominant taxa in the stroma of S. bambusicola. I t was a surprising finding that Pseudomonas isolates such as P. putida, P. fulva and P. parafulva exhibited a general capacity to stimulate the secretion of HA in the mycelium of S. bambusicola. P. fulva SB1 showed the most significant effects on HA accumulation . When live SB1 at 100 cells /mL was applied to the submerged culture of S. bambusicola on day 4, the HA content was most effectively enhanced 2.47-fold (17.43 mg/g DW versus 7.06 mg/g DW in control) on day 8. The results provide a reference of interaction between fruiting-body associated bacterium and medicinal fungus S. bambusicola. Our study will facilitate its potential application as a novel bacteria-fungi co-culture strategy for mass production of pharmaceutically important metabolites.
Acknowledgement: The study is supported by the NNFC (No. 81473183 and 81773696) and the Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX17_2042) .
Keywords: Shiraia bambusicola ; fruiting body; hypocrellin; Pseudomonas; elicitation
References:
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[2] Sun CX, Ma YJ, Wang JW. 2017. Enhanced production of hypocrellin A by ultrasound stimulation in submerged cultures of Shiraia bambusicola. Ultrasonics Sonochemistry 38: 214-224