16:00 - 18:00
Room: Poster Area - Poster Shed
Poster Presentation
Phytochemical characterization and anti-dementia effects of major constituents from the seeds of Zizyphus jujuba var. spinosa
Hye Min Kim 1, Haneul Kim 1, Jun Hee Jang 1, Sanghyun Lee 2, Dong Hyun Kim 3, 4, Jong Hoon Ryu 5, 6, In Ho Jung 1, 5
1 Daehwa Pharmaceutical Co., Ltd., Seongnam-si 13488
2 Department of Integrative Plant Science, Chung-Ang University, Anseong 17546
3 Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan 49315
4 Institute of Convergence Bio-Health, Dong-A University, Busan 49315
5 Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul 02447
6 Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul 02447

The seeds of Zizyphus jujuba Mill. var. spinosa (Bunge) H. H. Hu ex H. F. Chow ( 枣仁 ; family Rhamnaceae) has been used as traditional medicine to treat sedation and hypnosis in Eastern Asia. Our previous studies have indicated that the ethanolic extract of Z. jujuba var. spinosa (DHP1401) improves memory impairment, a common symptom of various neurological diseases. In this study, the chemical composition of DHP1401 has been isolated and characterized. Additionally, we investigated the anti-dementia effects of major components of DHP1401 on memory impairment by scopolamine, a muscarinic receptor antagonist. Phytochemical constituents were isolated from the dichloromethane, ethylacetate, n-butanol fractions of DHP1401 using repeated chromatography and prep-HPLC systems to yield 13 compounds. The isolated compounds were elucidated as pinostrobin (1), 6-demethoxyafromosin (2), diosmetin (3), buddlenol C (4), ficusequilignan A (5), ceanothic acid (6), alphitolic acid (7), naringenin (8), 6'''-feruloylspinosin (9), nicotiflorin (10), swertisin (11), spinosin (12), magnoflorine (13) by spectroscopic analysis. Among them, swertisin (11) and spinosin (12), which are well known as the major components of Z. jujuba var. spinosa, attenuated the scopolamine-induced cognitive deficit in passive avoidance task. Further studies will focus on the profiling of components in DHP1401 and the characterization of the constituents responsible for the anti-dementia effects.


Reference:
Poster Session-PO-57:
Session:
Poster Presentation-1
Presenter/s:
Hye Min Kim
Presentation type:
Poster presentation
Room:
Poster Area - Poster Shed
Date:
Monday, 27th August, 2018
Time:
16:00 - 18:00
Session times:
16:00 - 18:00