16:00 - 18:00
Room: Poster Area - Poster Shed
Poster Presentation
Phytochemical composition and antioxidant evaluation of Pelargonium sidoides DC. root extract and proanthocyanidins
Lina Raudone 1, Maija Dambrova 2, Elina Makarova 2, Helena Kazoka 2, Nijole Savickiene 1
1 Department of Pharmacognosy, Lithuanian University of Health Sciences, Kaunas
2 Latvian Institute of Organic Synthesis, Riga

Pelargonium sidoides roots have been used for medicinal purposes in various traditional medicine systems. Proanthocyanidins (PACN) are bioactive constituents of Pelargonium sidoides root extracts (PSRE) and possess antioxidant, anti-inflammatory, antibacterial properties [1]. PACN enriched extracts inhibit biofilm formation and adhesion of periodontopathogenic bacteria, inhibit proteolytic activities of bacteria [2]. PACNs could be perspective bioactive compounds beneficial in development and progression of periodontal diseases. The aim of this study was to determine proanthocyanidin composition in PSRE and collected PACN fraction. UPLC-MS analysis was used for the determination of proanthocyanidin profile and the estimation of the mean degree of polymerization (mDP) for the oligomers and polymers in PCAN. Reducing and radical scavenging activities of the extracts were determined using FRAP and ABTS assays, respectively. Five prodelphinidin derivatives were determined in the PSRE and PACN fraction (from dimers to hexamers). The calculated mean degree of polymerization for analysed PCANs sample was 6. The determined radical scavenging and reducing activities were 3747.75 ± 534.56 µmol/g (PSRE) and 4472.75 ± 707.71 µmol/g of TE (PACN) and 3210.17 ± 421.21 (PSRE) and 4006.13 ± 685.75 (PACN), respectively. The PACN fraction consisting of prodelphinidin oligomers showed significantly (p<0.05) higher Trolox equivalent antioxidant activity compared to PSRE. PACNs could be perspective for functionalization drug delivery systems of local action with natural active substance for treatment of periodontal diseases.

Acknowledgement: The PELARGODONT Project (“Engineering and functionalization of delivery system with Pelargonium sidoides biologically active substance on inflamed periodontal surface area”) is financed under the M-ERA.NET 2 Programme, which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 685451.

References: 1. Kolodziej, H. (2008) Planta Med 74: 661–666. 2. Palaska, I et al., (2013) Eur J Pharmacol 720:77-83.

Keywords: Pelargonium sidoides, proanthocyanidins, antioxidant, UPLC-MS.


Reference:
Poster Session-PO-67:
Session:
Poster Presentation-1
Presenter/s:
Lina Raudone
Presentation type:
Poster presentation
Room:
Poster Area - Poster Shed
Date:
Monday, 27th August, 2018
Time:
16:00 - 18:00
Session times:
16:00 - 18:00