Cinamomum camphora is proved to possess a lot of biological activities, including antifungal, antioxidant, antibacterial, anti-allergic, and anti-inflammatory activities [1-2] . However, the bioactive compounds responsible for the anti-inflammatory effect of leaves have not been yet determined. The objective of the present study was to isolate these bioactive compounds by bioassay-guided isolation technique and to determine the mode of action of isolated compounds in LPS-induced macrophages. We demonstrated that ethyl acetate fraction significantly decreased the production of inflammation mediator nitric oxide (NO) and inflammatory cytokines tumor necrosis factor PGE2 and TNF-α in a dose-dependent manner (10, 30, 100 µg/ml). In addition, Sevens compounds ursolic acid (1), β-sitosterol (2), trametenolic acid (3), (-)-sesamin (4), dimethylmatairesinol (5), oleanolic acid-3-O-β-D-glucoside (6), and quercetin-3-O-β-D-glucoside (7). were isolated from ethyl acetate fraction , among them, three compounds, ursolic acid (1), trametenolic acid (3) and oleanolic acid-3-O-β-D-glucoside (6) were reported for the first time in Cinamomum camphora . NO and inflammatory cytokines ( PGE2 and TNF-α ) secretion indicated that ursolic acid (1), β-sitosterol (2), trametenolic acid (3) and oleanolic acid-3-O-β-D-glucoside (6) showed a more outstanding anti-inflammation potential at non-toxic concentrations (10, 30, 100 µM) than the other four compounds.
References. 1. Jiang H, Wang J, Song L, et al. Molecules 2016, 21(4): 423-432.
2. Pragadheesh V S, Saroj A, Yadav A, et al. Ind Crop Prod , 2013, 49: 628-633.
Acknowledgements: This work was funded by the Science Foundation for Young Doctors of Jiangxi academy of science (2016-YYB-07), the Science and Technology Major Project Foundation of Jiangxi Academy of Sciences (2018- YZD2-18) . Foundation of Key Project of Research and Development Program of Jiangxi Province (No. 20171BBH80017)