GA 2017

Quinolizidine alkaloids are naturally-occurring compounds in Lupinus plants [1]. Wild species from this genus have a wide distribution in Colombia, but they have been slightly studied at bio and phytochemical level. According to this fact, the present work is intended to make a contribution to the physiological and metabolic study of the native plant Lupinus bogotensis on the basis of quinolizidine profiles. Most of these compounds are produced as part of the induced response against the herbivore-mediated attacks [2] and stimulation depending on environmental and stress conditions [3], so their characterization and variability is important to be evaluated as a potential source for bioactive quinolizidines. For this reason, the variation of L. bogotensis-derived alkaloidal profiles was evaluated on comparing two propagation environments, i.e., greenhouse and in vitro, and two developmental stages (i.e., first and second pair of leaves). Dry and fresh plant material (n=40) were extracted (using conventional alkaloidal acid-base protocol) and resulting extracts were then analyzed by GC-MS. Profiles exhibited an alkaloid-enriched composition involving quinolizidines such as lupanine as the main metabolite. Presence of other alkaloids at lower abundance was also detected such as nutalin, 5,6-dehydrolupanine, and sparteine. Fresh, greenhouse-grown seedlings were discriminated by an OPLS-DA model due to a greater presence of alkaloids while in-vitro-propagated seedlings exhibited lower alkaloidal content. Some quinolizidines were consequently correlated for each stage as developmental biomarkers. Hence, previous results allowed establishing specific stages for selective quinolizidine production, which could be also used in the future, e.g., for antimicrobial purposes due to its toxic properties. Work derived from Project IMP-CIAS-2293 financed by Vicerrectoría de Investigaciones at UMNG - Validity 2017. References: [1] Ganzera M, Krüger A, Wink M. J Pharm Biomed Anal 2010; 53:1231-1235; [2] Wink, M. Phytochemistry, 2003; 64:3–19; [3] Pichersky, E., Gang, D. R. Trends Plant Sci 2000; 5:439–45.