Matrix metalloproteinases are responsible for breaking down extracellular matrix and MMP-8 is an interstitial collagenase, modulates TNF-α, which may explain neuroinflammation [1]. It plays crucial roles on various cancer types and diseases such as obesity and rheumatoid arthritis [1-2]. In such diseases, inhibition of MMP-8 could be a part of treatment strategy, so we aim to inhibit MMP-8 by Rumex crispus extracts. Rumex species (Polygonaceae) contain anthranoids, tannins, flavonoids and naphthalenes as secondary metabolites and have various biological effects [3].
Standardized n-hexane, dichloromethane, ethyl acetate, ethanol and ethanol:water (70:30) extracts were prepared from roots, leaves and fruits and inhibitory effects on MMP-8 were investigated. NNGH was used as potent MMP-8 inhibitor. Inhibitor activities of all extracts were measured in 50 μg/mL, 100 μg/mL, 200 μg/mL, 300 μg/mL, 400 μg/mL and 800 μg/mL concentrations at 412 nm. The aqueous alcoholic extracts showed the highest inhibitory effect among other extracts at all concentrations. Inhibition values were 86.25% for root extracts, 84.48% for leaf extracts and 87.30% for fruit extracts, while NNGH was 93.88%. This study shows that aqueous alcoholic extracts of Rumex crispus may be potential novel MMP-8 inhibitors.
Acknowledgements: This study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) (Project No:SBAG-214S147)
Keywords: Matrix metalloproteinase, Rumex crispus, MMP-8 inhibitor
References:
[1] Lee EJ, Han JE, Woo MS, Shin JA, Park EM, Kang JL, Moon PG, Baek MC, Son WS, Ko YT, Choi JW, Kim HS. J. Immunol 2014; 193(5): 2384-2393
[2] Foda HD, Zucker S. Drug Discov Today 2001; 6(9): 478-482
[3] Vasas A, Orban-Gyapai O, Hohmann J. J Ethnopharmacol 2015; 175: 198-228