delivery with in vivo studies
The purpose of this study is to prepare polyamide nanocapsules containing Elaeagnus angustifolia L. using
an emulsion diffusion technique with in vivo studies. Diethylenetriamine (DETA) was used as the encapsulating
polymer in acetone ethyl acetate (EA) and dimethyl sulfoxide (DMSO) as the organic solvents,
Tween 80, Tween 60, Tween 20, Gelatin, and sodium lauryl sulfate (SLS), in water as the stabilizers. Two
ratios of organic to aqueous phases were used with each solvent and stabilizer. The nanocapsule E. angustifolia
were obtained with a mean diameter of 60–180 nm, zeta potential of
−11.3 mV, Particles Dispersion
Index (PDI) of 0.2, and a high encapsulation efficiency of 0.98%. Acetone was superior to EA and DMSO,
and Tween 20 was superior to Tween 80, Tween 60, Gelatin and SLS in obtaining smaller nanocapsules.
An organic of aqueous phase ratio of 1:5 was shown to be more suitable for the smaller nanocapsules.
Finally, nanocapsules containing E. angustifolia and calcium carbonate as well as calcium tablets available
on the market, were administered to rats for 3 weeks, and the calcium levels were measured in their
blood as well as compared to a normal control group. The results showed that the E. angustifolia group
has a higher level of calcium in their blood.