The study aims to develop a new screening technique for the detection of inhibitory metabolites from local mangrove actinomycetes toward β-lactamase (1). This new screening technique is believed to contribute a novel bioactivity. The oxacillin-resistance Staphylococcus aureus ATCC 43300 was used as test organism and the presence of β-lactamase enzyme from this pathogen was detected by using nitrocefin (2). Among 41 marine-derived actinomycetes, two isolates designated as KMS1 and THH1 consistently exhibited the production of metabolites that can act as an inhibitor agents. For that reason, one of the active isolates, KMS1 was grown in a large volume to extract and check the presence of metabolites as inhibitors of β-lactamase. This confirmation test was effectively done by using oxacillin-susceptible Staphylococcus aureus ATCC 25923 and penicillinase. Therefore, this study has successfully developed a new screening technique for a novel β-lactamase inhibitor bioactivity from local mangrove actinomycetes.
Acknowledgements: The authors thank the Institute of Graduate Studies and Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM) Shah Alam, Selangor for providing the funds and facilities for this study. The credit also goes to Dr. Mohamad Ali Yusuf (UiTM Perak Branch, Tapah Campus) who helped in the editing work.
Keywords: β-lactamase inhibitor, mangrove actinomycetes
References:
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