16:00 - 18:00
Room: San Francisco
Poster session
Sesquiterpenes from Neurolaena lobata with antiproliferative activity
Vasas Andrea 1, Lajter Ildikó 1, Forgó Péter 1, Kúsz Norbert 1, Bózsity Noémi 2, Zupkó István 2, Nagy Nikolett 2, Krupitza Georg 3, Frish Richard 4, Hohmann Judit 1, 5
1 Institute of Pharmacognosy, University of Szeged, Szeged, Hungary
2 Institute of Pharmacodynamics and Biopharmacy, University of Szeged, Szeged, Hungary
3 Clinical Institute of Pathology, Medical University of Vienna, Vienna, Austria
4 Institute of Ethnobiology, Playa Diana, San José/Petén, Guatemala
5 Interdisciplinary Centre of Natural Products, University of Szeged, Szeged, Hungary

Neurolaena lobata L. (Asteraceae) is a perennial herb native to tropical part of America. It has been used in traditional medicine for the treatment of different types of cancer, ulcers, inflammatory skin disorders and diabetes. The main constituents of the plant are sesquiterpene lactones, which can be responsible for its different pharmacological effects. Our previous studies revealed the presence of sesquiterpenes of the germacranolide, eudesmanolide and furanoheliangolide types, and some of them proved to be active against cancer and inflammation in vitro and in vivo.

In continuation of our work, further sesquiterpenes were isolated from N. lobata and their antiproliferative activity was investigated on human cancer cell lines (SiHa, HeLa and C33A, at 10 and 30 µM concentrations) using the MTT assay. The aerial parts of the plant were extracted with methanol. After evaporation, the extract was subjected to solvent–solvent partition with n-hexane and CH2Cl2. The CH2Cl2 fraction was chromatographed by a combination of different methods, including CC, VLC and PLC. The structure elucidation of the compounds was carried out by means of MS, 1D and 2D NMR spectroscopy.

The results allowed the identification of five sesquiterpenes, four of them esterified with isovaleric acid at C-8 or C-9. The new compounds are sesquiterpene lactones, the dimer lobatolide A, the calyculatolide derivative lobatolide B, and the germacranolide-type lobatolides C and D. Furthermore, one known guaiane-type sesquiterpene [(+)-aphanamol I] were also isolated from the plant. With the exception of the known compound, all component possessed remarkable antiproliferative activities (inhibition % ± SEM between 60.92 ± 1.73 and 96.45 ± 0.27) on at least two cell lines, at 30 µM concentration. Lobatolide A proved to be the most active.

Acknowledgement: This work was supported by GINOP-2.3.2-15-2016-00012, ÚNKP-16-3 and a János Bolyai Research Scholarship of the Hungarian Academy of Sciences.


Reference:
Mo-Poster Session 1-PO-69:
Session:
Poster Session 1
Presenter/s:
Judit Hohmann
Presentation type:
Poster presentation
Room:
San Francisco
Date:
Monday, 4th September, 2017
Time:
16:00 - 18:00
Session times:
16:00 - 18:00