16:00 - 18:00
Poster Session 1
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Anti-inflammatory and anti-tubercular properties screening of natural products from Plectranthus species
Marçalo Joana 1, Garcia Catarina 1, Custódio Luísa 2, Nicolai Marisa 1, Reis Catarina 1, 3, Rodrigues Maria João 2, Romagnoli Alessandra 4, Petruccioli Elisa 4, Goletti Delia 4, Monteiro Rodrigues Luís 1, Faustino Célia 5, Fimia Gian Maria 4, 6, Rijo Patrícia 1, 5
1 Universidade Lusófona’s Research Center for Biosciences and Health Technologies (CBIOS), Lisboa, Portugal
2 Centre for Marine Sciences (CCMAR), University of Algarve, Campus of Gambelas, Faro, Portugal
3 Biophysics and Biomedical Engineering Institute (IBEB), Faculty of Sciences, University of Lisbon (ULisboa), Lisbon, Portugal
4 National Institute for the Infectious Diseases “Lazzaro Spallanzani”, Rome, Italy
5 Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, University of Lisbon (ULisboa), Lisbon, Portugal
6 Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A.), University of Salento, Lecce, Italy

Medicinal plants of the Plectranthus genus are widely used in traditional medicine for their anti-inflammatory and anti-infective properties [1]. Previous reports [2,3] directed this study for the production and release of Nitric Oxide, and for the CFU analysis of Mycobacterium tuberculosis H37Rv (Mtb) growth inhibition, of Plectranthus spp. non-cytotoxic compounds.

The natural compounds isolated from Plectranthus spp. using bioassay-guided fractionation of extracts, were: 6β,7α-dihydroxyroyleanone and 6,7-dehydroroyleanone (P. madagascariensis); β-sitosterol, stigmasterol, oleanolic and ursolic acids (P. ecklonii); (13S,15S)-6β,7α,12α,19-tetrahydroxy-13β,16-cyclo-8-abietene-11,14-dione (P. porcatus); (11R*,13E)-11-acetoxyhalima-5,13-dien-15-oic acid, Plectrornatin C, 1,6-di-O-acetylforskolin and 1,6-di-O-acetyl-9-deoxyforskolin (P. ornatus); α-amyrin and β-amyrin (P. neochilus); chlorogenic acid (P. saccatus) and rosmarinic acid (all aqueous extracts).

The compounds were unable to reveal in vitro anti-inflammatory activity neither in LPS-stimulated RAW 264.7 cells nor in the S-nitroso-N-acetylpenicillamine assay. Nonetheless, regarding the Mtb growth inhibition, one semi-synthetic halimane derivative (11R*,13E)-halima-5,13-diene-11,15-diol) previously prepared [4], revealed promising results (2.1x105 CFU/mL), with an effect similar to the anti-tubercular drugs ethambutol (2.0x105 CFU/mL) and isoniazid (1.2x105 CFU/mL).

To the best of our knowledge, this report is the first scientific validation on Plectranthus spp. isolated compounds concerning their anti-inflammatory activity for NO production and release, and concerning their anti-mycobacterial activity for Mtb growth inhibition. Further studies regarding other inflammatory mediators are under study.

[1] Lukhoba CW, Simmonds MS, Paton AJ. J. Ethnopharmacol 2006; 103: 1–24;

[2] Rijo, P, Simões MF, Francisco AP, Rojas R, Gilman RH, Vaisberg AJ, Rodríguez B, Moiteiro C. Chem. Biodivers 2010; 7: 922–932;

[3] Rijo, P, Gaspar-Marques C, Simões MF, Jimeno ML, Rodríguez B. Biochemical Systematics and Ecology 2007; 35: 215–221.

Reference:
Mo-Poster Session 1-PO-87:
Session:
Poster Session 1
Presenter/s:
Joana Marçalo
Presentation type:
Poster presentation
Room:
San Francisco
Date:
Monday, 4th September, 2017
Time:
16:00 - 18:00
Session times:
16:00 - 18:00
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